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To understand molecular mechanisms of the dual and triple agonism conferred by tirzepatide and peptide 20, we determined five cryo-electron microscopy (cryo-EM) structures, including GIPR and GLP-1R bound with tirzepatide and GIPR, GLP-1R and GCGR bound with peptide 20, all in complex with G s proteins at global resolutions of 3 simm simm simm simm.ac.cn (M.-W.W) Supplemental files (seven PDBs and their maps) updated

Specifically, hepatoma cells with low expression of GOT2 showed a high dependence on Gln metabolism by increasing Gln metabolism, nucleotide synthesis, and glutathione synthesis to support cellular antioxidants (Fig

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