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Fluorescent sensors are widely developed for the detection of thiol-containing biological molecules owing to the superior sensitivity, fast response and unrivalled spatio-temporal resolution 14

The concentration of Mouse GSH in the samples is then determined by comparing the OD of the samples to the standard curve

Therefore, future research is needed to focus and continue to explore optimal ratios for enhancing reproductive performance in roosters, as an imbalance can negatively impact semen quality (Clment et al., 2012)

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