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PEER REVIEW The peer review history for this article is available at Supporting information ACKNOWLEDGEMENTS The authors would also like to acknowledge Caroline Spencer (Rx Communications, Mould, UK) for medical writing assistance with the preparation of this manuscript, funded by Eli Lilly and Company

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l-valine stimulates GLP-1 secretion in perfused rat small intestines and GLUTag cells In isolated perfused small intestines ( n = 8) from Wistar rats, luminal infusion of l-valine (50 mM) powerfully stimulated GLP-1 secretion in a control experiment with repeated stimulation ( p 0.05