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Nevola, R., et al

A Dionex Ultimate 3000 high-performance liquid chromatography system and Q Exactive quadrupole orbitrap mass spectrometer (Thermo Fisher) were used to analyze the UBE2m-derived peptides

Each has somewhat different kinetics with different parameters: UGT3 has simple Michaelis-Menten kinetics

Timeline include: 24 to 48 hours: early improvement 3 to 5 days: major reduction 1 to 2 weeks: final settling Most patients require more than one session after a few weeks, especially if a large amount of filler was used and migration happened

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