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Without adequate glutathione, these toxins can linger, causing damage to liver cells and placing a burden on the rest of the body

doi: 10.1136/jcp.2009.072561 114 KomaroffAL

Sequencing libraries were prepared using the NEBNext UltraExpress RNA Library Prep Kit for Illumina (NEB, E3330) according to the manufacturer protocol and sequenced on DNBSEQ-G400, MGI (150 bp paired-end, minimum 100 mil reads per condition, 2 biological replicates)

The microbiota toxic substances lead to macrophage activation in the Kupffer cells, the livers resident macrophages, become activated by LPS and other microbial products, producing ROS and pro-inflammatory cytokines

The primer sequences used for amplification of the studied genes were taken from previous published studies [33, 34]