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Finally, 50 L of Stop Solution was added to each well and mixed, followed by measurement of absorbance at 450 nm in a SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA)

LPA (unspecified species) promotes epithelial-mesenchymal transition and migration while attenuating EGFR-TKI efficacy, effects that are reversible by disrupting LPA-LPAR signaling (97)