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It is studied because copper is involved in several biological processes linked with tissue structure, connective tissue, collagen systems and extracellular matrix regulation

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NCTC1469 cells, a normal mouse hepatocyte cell line, were obtained from the American Type Culture Collection and were cultured in DMEM containing 10% (v/v) fetal horse serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere

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AcAcCoA, acetoacetyl coenzyme A