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Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Biowest, Nuaille, France), 100 g/mL streptomycin, and 100 U/mL penicillin (Thermo Fisher Scientific) at 37C in a humidified atmosphere containing 5% CO 2

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In the PPAR agonist experiments, mice were intraperitoneally injected with WY14643 at 50 mg/kg (dissolved in 100 L of 10% DMSO and 90% corn oil) daily for 5 days

Focus on comprehensive care Medication is just one piece of your wellness plan lifestyle, nutrition, and behavioral changes remain the foundation of effective long-term weight management