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The next day, the cells were incubated for 1 h with Alexa Fluor 488-conjugated goat anti-rabbit IgG (A21245) secondary antibody

This timing takes advantage of the bodys natural post-overnight fast state when insulin levels are low and fatty acid mobilization is already elevated

1997 Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade Cell 91 : 479489 Ghibelli L, Coppola S, Fanelli C, Rotilio G, Civitareale P, Scovassi AI, Ciriolo MR

more recently, much attention has been focused on plants and their associated phytomicrobiome (Compant et al., 2019)

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