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Reagent A with 0.64-mM GSH (90 l) was diluted to a fully collected reaction mixture (104 l) by 1.15 (5) times to a GSH concentration of 0.55385 mM (i.e., working concentration of GSH)

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The non-coenzyme action of thiamine in neurotransmission is further supported by the action of the thiamine analog oxythiamine, whose diphosphorylated derivative (oxyThDP), formed by TPK in vivo , is an antagonist of the coenzyme action of thiamine