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For HiBiT:LgBiT luciferase live-cell complementation assays, 15 K N2A cells were seeded in 96-well plates and transfected with 33 ng per well pLX304_CMV::mCherry-LgBiT-FLAG-NES (Addgene #199715) 24 h after seeding

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Mechanisms of heterogeneous distribution of monoclonal antibodies and other macromolecules in tumors: Significance of elevated interstitial pressure

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[6] These analyses are useful, but they are the weakest tier for a two-drug verdict: indirect comparisons inherit every difference between the underlying trials and cannot substitute for a randomised head-to-head