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In brief, male mice were deeply anaesthetized with an intraperitoneal injection of 7% chloral hydrate and transcardially perfused with a modified ice-cold artificial cerebrospinal fluid (aCSF) (described below)

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At present, the most studied SIRT agonists mainly target SIRT1, SIRT3, and SIRT6, and these activators upregulate the expression and activity of these enzymes to mediate the related pathways, thus playing a protective role in the kidney (Table 3)