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Cryo-EM data were collected on a Tecnai Arctica (FEI, Hillsboro, OR) at 200 kV, utilizing an energy filter and a direct electron detector (Gatan, Pleasanton, CA) operated by EPU software (Thermo Fisher Scientific, Waltham, MA)
Pen Dosage Chart Dosage & Protocols Variations Standard Research Protocol Dose: 5 10 mg (= 50100 clicks) Duration: 2 3 weeks Frequency: Daily Cycle Interval: 4 6 months off before repeating Goal / Description: Baseline protocol used in telomerase activation and cellular aging models
IL-1 is a well-established upstream regulator of age-associated inflammation and induces downstream cytokines including IL-6 and TNF 4,49