A 20 l mix, containing 8 l of cDNA sample, 10 l of SYBR Green PCR Master Mix (Thermo Fisher Scientific), and 2 l of 10 mM forward primer and reverse primer mixture was used to performed Quantitative RT-PCR with a StepOne Plus Real-time PCR system (Applied Biosystems, Foster City, CA, United States)
Adrenergic pharmacology: focus on the central nervous system
These enzymes are responsible for metabolizing 70% of clinically prescribed drugs 22,23 , establishing this timepoint as pivotal for investigating drug metabolism, interactions, and toxicity
The clinical characteristics of PD comprise motor disorders, such as static tremor, muscle stiffness, motor retardation, gait disorders and non-motor symptoms, such as reduced sense of smell, mental disorders, sleep disorders, cognitive disorders 3
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