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High antioxidant intake may put additional load on the kidneys for excretion

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RNA isolation and real-time quantitative PCR Total RNA was isolated with E.Z.N.A. Total RNA Kit I (Omega Cat#R6834-01) and reverse transcription (RT) reactions were performed with High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Cat#4368814) according to manufacturers protocol

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