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PMCID: PMC11817741

Protein concentration was determined using a BCA Reducing Reagent compatible assay kit (catalog: 23250, Thermo Fisher Scientific)

The observation in this study in hepatocytes that protein-SSG formation by either GSSG accumulation or treatment with a direct S -glutathionylation induction agent resulted in IKK--SSG formation, leading to its suppression, was supported by a previous study reporting that S -glutathionylation was associated with suppressed IKK- activity in epithelial cells 41

The relevance of the cytosolic production of GSH was characterized by complementing mutant plants deficient in GSH2 with a wild-type GSH2 exclusively targeted to the cytosol