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The major challenge of the in vitro systems yet to be solved is to cover the whole complexity of the MASLD pathogenesis including the crosstalk between the liver, adipose tissue, and gut (Ramos et al

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Unfortunately, this compensatory surge is futile because the downstream clearance pathwaysspecifically the enzymes mitochondrial superoxide dismutase (SOD2) and glutathione peroxidase 4 (GPX4)are severely dysfunctional