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A relationship between long-term metformin use and vitamin B12 deficiency has been long discussed, and many readers will have come across this in clinical practice

However, these anatomical and behavioral differences dampen with age, though long-term changes in genomic signaling characterized by increased cyp19a1b expression, decreased ER protein, and decreased thrb expression persist to adulthood

Published murine studies used 25-50 mg/kg intraperitoneal injection once or twice daily