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MTRR The B12 used by MTR eventually becomes oxidised , and needs to be reactivated via methylation by another enzyme - MTRR (Methionine synthase reductase), using SAMe as a methyl donor

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In brief, brains and spinal cords were rapidly dissected in cold 0.15 M sodium cacodylate pH 7.4 containing 4% paraformaldehyde (PFA) and 0.5% glutaraldehyde