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After three washes with PBS, the sections were pre-incubated in blocking solution (5% serum albumin, 2% normal goat serum, and 0.3% Triton X-100 in PBS) for 20 min at room temperature, then incubated with an anti-c-Fos rabbit antibody (#sc-52, 1:2000

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Both tests came back negative: AOD9604 did not cause mutations in bacteria and did not damage chromosomes in mammalian cells

10.1111/j.1432-1033.1983.tb07429.x 35 FisherA